Ursolic acid(UA) was isolated from Olednlandiae diffusae Herba, one of the commonly used medicinal herbs for the treatment of cancer. IC50 of UA against cancer cell lines as SNU-1, B160Fo, SK-OV3, HCT15, XF498, SK-MEL and A549 was
6ug/ml,
4.4ug/ml, 4.5ug/ml, 4.6ug/ml and 4.2 ug/ml respectively suggesting cytotoxicity against cancer cells. DNA fragmentation was expressed from the concnetration of 5.5ug/ml of UA by agarose electrphoresis. In the observation of
morphological
changes by p0hase contrast microscope, SEM and TEM. cell injury and condensation of cytoplasm from nucleus began 4 hr after UA treatment, fragmentaion of nucleus and injury of cell membrane was shown 24 hr after UA treatment with SNU-1
cells.
Aurin tricarboxic acid as endonuclease inhibitor, and nicotinamide as poly(ADP-ribose) polymerase inhibitor protected over 50% of cytotoxicity of UA aginst SNU-1 was at the concentrations of 3 M and 300 M respectively suggesting UA acts
on
nucleus. These results suggest that UA had antimetastatic effect and induced apoptosis
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